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Design Primer
Type of query:
Range
Seq
Enter FASTA sequences:
e.g.,
example
Varieties:
02428
9311
Basmati1
CG14
CN1
D62
DG
DHX2
FH838
FS32
G46
G8
G630
II32
IR64
J4155
KY131
Lemont
LJ
NIP(MSU)
N22
NamRoo
Y3551
R498
R527
S548
TM
Tumba
WSSM
Y58S
Kosh
YX1
ZH11
Chromesome:
Chr1
Chr2
Chr3
Chr4
Chr5
Chr6
Chr7
Chr8
Chr9
Chr10
Chr11
Chr12
Start position:
End position:
Parameter Selection:
Sequence target:
Product Size Ranges:
Optimal primer size:
Max primer size:
Min primer size:
Optimal primer TM:
Max primer TM:
Min primer TM:
Optimal GC percent:
Max GC percent:
Min GC percent:
Max #N's accepted:
Max Poly-X:
Max Internal Poly-X:
Max Self Complementarity:
Max 3' Self Complementarity:
Max Pair Complementarity:
Max 3' Pair Complementarity:
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